FastQCFastQC Report
Thu 30 Aug 2018
ChIPseq-RXRa-TCP-rep-1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-RXRa-TCP-rep-1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17241628
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT185582510.763629745404552TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA11654806.7596864982819485TruSeq Adapter, Index 13 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT205770.11934487856947151TruSeq Adapter, Index 13 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)