FastQCFastQC Report
Thu 23 Aug 2018
RXRa-CTL-rep-1_2_3_4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXRa-CTL-rep-1_2_3_4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72282320
Sequences flagged as poor quality0
Sequence length100-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT2135350.2954180220004007TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA1100840.15229726992714127TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTG573200.038.22629532-33
AGTCACG1149400.037.14385628-29
TATCGGA79850.036.9740871
TCACGAG658000.036.76975330-31
ACGGCTA514050.035.1033732-33
CGGAAGA3209100.034.1733324
TCGGAAG3235100.033.7857553
ATCGGAA3292600.033.2404562
GAGCACA3388500.032.1888779
GGATATC740650.032.17822338-39
TCACGGC563100.031.99783530-31
GATCGGA3327100.031.6019521
AGAGCAC3571350.030.6160628
AATCTCG459200.029.94593840-41
GAAGAGC3671800.029.8362586
CGGCTAC512000.027.73375332-33
CGTCTGA2450200.027.2862416-17
AAGAGCA4043250.027.2351157
ATATCTC890750.027.19669340-41
TATCTCG649750.026.7622440-41
Produced by FastQC (version 0.11.2)