FastQCFastQC Report
Thu 23 Aug 2018
ChIPseq-CAR-TCP-rep-1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-TCP-rep-1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38893598
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT4578801.1772631578081307TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA1036600.26652201218308474TruSeq Adapter, Index 25 (97% over 44bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530810.13647747374773606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG1096750.072.197483
ATCGGAA1117550.071.4182362
CGGAAGA1126950.070.594654
GATCGGA1054100.068.932371
TATCGGA74800.061.736271
GAGCACA1412550.055.3044939
AGAGCAC1452850.053.8312348
GAAGAGC1481300.053.0359046
GGAAGAG1710950.047.333865
AAGAGCA1706850.046.3843967
CGTCTGA1108550.042.45610416-17
CACGTCT1136300.041.30060214-15
CACACGT1157400.040.26444212-13
CTCGTAT1044450.038.99344-45
ATCTCGT1063750.038.47904642-43
CGTATGC1093600.037.71804446-47
TGCCGTC1076600.037.55317350-51
TATGCCG1121850.036.86892748-49
ACTGATA1042500.036.7853934-35
CCGTCTT1120950.036.62932252-53
Produced by FastQC (version 0.11.2)