FastQCFastQC Report
Thu 23 Aug 2018
CAR-TCP-rep-2_6_7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAR-TCP-rep-2_6_7.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68358821
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC6037410.8831939918916974TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4273200.6251131803458109TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT1754850.037.28038850-51
TGCCGTC1769350.037.03614848-49
CGTATGC1805850.036.49175344-45
CTCGTAT1801000.036.41068342-43
TATGCCG1823700.036.16798846-47
ATCTCGT1876400.034.9175640-41
TGTAATC1599500.034.84233536-37
TAATCTC1626400.034.39561538-39
TGCTTGA1938950.033.16535658-59
CTTGTAA1693600.033.003234-35
ACCTTGT1743350.032.12212432-33
TCACCTT1878150.029.83758230-31
GTCTTCT2278850.028.8684152-53
AGTCACC2509050.027.95468728-29
CTTCTGC2511750.026.32775154-55
TCTGCTT2524400.026.18163956-57
CCAGTCA2726250.026.02067426-27
CGTCTGA2807750.025.47778916-17
CTTGAAA2633300.024.5580460-61
AATCTCG1346700.024.25406338-39
Produced by FastQC (version 0.11.2)