FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-RXRa-TCP-rep-2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-RXRa-TCP-rep-2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11891150
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303060.2548618089924019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8500.013.97531544
ACGACGC3051.8189894E-1213.70315134
ATCGGAA7550.012.82013244
CGACGCT3309.1677066E-1011.33187335
CACGACG3003.115565E-810.99858233
AGATCGG11950.010.12430343
GCGACCA4703.271998E-88.4244453
CCGATCT5752.055458E-108.4165199
TCCGATC5302.4783367E-77.4709238
TACACGA6255.3274016E-76.687587731
CGGCGAC4353.966807E-46.6026831
CGACCAC6001.9390482E-66.5991494
GGCGACC7352.919478E-86.5841852
TAGATCG5452.234231E-56.45818244
CTACACG7409.525833E-65.64837130
ACACGAC6707.361329E-55.581369432
TTCCGAT7601.4916155E-55.49942977
Produced by FastQC (version 0.11.2)