FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-RXRa-TCP-rep-1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-RXRa-TCP-rep-1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17241628
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT185582510.763629745404552TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA11654806.7596864982819485TruSeq Adapter, Index 13 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT205770.11934487856947151TruSeq Adapter, Index 13 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA78550.059.5533561
GAATCGG42450.052.4753651
TGCCGTC3526000.051.16977750-51
TATGCCG3580600.050.8962148-49
CGTATGC3625350.050.40766546-47
CCGTCTT3787050.046.6779752-53
AGATCGG3145300.045.141951
TGCTTGA3990550.043.27110360-61
CTTGAAA3951100.042.62831562-63
CGGAAGA5592650.042.513624
CAATCTC4283600.042.36635240-41
GTCTTCT4285050.042.29823754-55
TCGGAAG5631800.042.200593
CTCGTAT4362950.042.1828544-45
TCTGCTT4351750.042.08502658-59
ATCGGAA5675300.042.0653762
AACAATC4332350.041.929338-39
GAGCACA5631100.041.8423549
GAAGAGC5658750.041.828356
ATCTCGT4393600.041.82520742-43
Produced by FastQC (version 0.11.2)