FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-RXRa-CTL-rep-1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-RXRa-CTL-rep-1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23601924
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT10579844.482617603547914TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA2780621.1781327657863825TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA37500.052.9182241
CGGAAGA2365400.049.926014
GAGCACA2381750.049.7447059
TCGGAAG2390950.049.301183
ATCGGAA2419700.048.8579832
AGAGCAC2438950.048.6558958
GAAGAGC2478950.047.9282346
GATCGGA2424550.047.6580051
AAGAGCA2586000.045.9460337
GGAAGAG2658900.044.439975
CTCGTAT1687600.042.96208644-45
TGCCGTC1688200.042.7779550-51
CCGTCTT1690300.042.71722452-53
CGTATGC1703750.042.64382646-47
TATGCCG1709350.042.464548-49
ATCTCGT1706950.042.43074842-43
AACAATC1750950.041.0925938-39
CAATCTC1753400.041.08443540-41
TGCTTGA1748350.040.9636560-61
AGTCAAC1754600.040.7060234-35
Produced by FastQC (version 0.11.2)