FastQCFastQC Report
Sun 6 Aug 2017
RXRa_All_Reps_Combined.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXRa_All_Reps_Combined.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169617351
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG38750.016.63585912
GCGTCGT39050.016.3384611
AGCGTCG43400.015.30922410
GATCGGA59150.011.7627861
ATCGGAA77100.09.2241432
GTCGTGT76150.08.60972113
GAGCGTC83600.08.421319
TCGTGTA80900.08.15839114
CGTGTAG89750.07.94217115
GTCGCCG58500.07.070398344
TGGTCGC64800.06.654365542
GTGGTCG71450.06.61982241
AGAGCGT108550.06.58721888
AAGAGCG124100.06.31142437
GATCTCG84550.06.24259334
TCGGAAG127550.05.97270353
CGGAAGA155100.05.08203554
Produced by FastQC (version 0.11.2)