FastQCFastQC Report
Sun 6 Aug 2017
RXRa_All_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXRa_All_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169617351
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA59100.011.8006271
CTCGTAT63900.010.39680344
TCTCGTA64050.09.92607543
ATCGGAA73300.09.7545922
CGTCTGA97050.07.933925616
ACACGTC111050.06.874078813
ATCTCGT104350.06.472299642
ACGTCTG121600.06.313975315
CCGTACG18300.06.011287733
TCGGAAG130050.05.75173
CGTACGT20400.05.500262734
Produced by FastQC (version 0.11.2)