FastQCFastQC Report
Sun 6 Aug 2017
G155-M8-Index27_ATTCCT-_ACB6A7ANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG155-M8-Index27_ATTCCT-_ACB6A7ANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20973726
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4000.015.40082512
AGCGTCG4450.014.83190210
GCGTCGT4300.014.32593911
GATCGGA6600.010.341711
TCGTGTA9600.07.562616314
GTCGTGT8900.07.41603913
ATCGGAA9050.07.29871272
GAGCGTC9950.07.0756049
CGTGTAG11405.456968E-126.1754915
AGAGCGT13652.1827873E-115.4803178
TGGTCGC7701.853641E-55.428486342
GTCGCCG7202.1684634E-45.194499544
Produced by FastQC (version 0.11.2)