FastQCFastQC Report
Thu 6 Jul 2017
RXR_TCPO_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRXR_TCPO_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69242490
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA21700.010.2399791
TCGTATG23850.09.22381243
CTCGTAT24700.08.55039142
CGTATGC26250.08.2127744
TCTCGTA25150.08.13512441
ATCGGAA30800.07.28596642
CGTCTGA37800.06.169223316
ATCTCGT39800.05.63819140
ACGTCTG46300.05.369190715
ACACGTC45500.05.27010113
TCGGAAG49750.05.12980373
Produced by FastQC (version 0.11.2)