FastQCFastQC Report
Tue 26 Mar 2019
SRR3928596_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3928596_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27243611
Sequences flagged as poor quality0
Sequence length68
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTC1869270.6861315117148017No Hit
ATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGG1292420.4743937945671005No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1009290.3704685109473924No Hit
GCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACC770970.2829911203768105No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGT760210.27904157051721223No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCC737720.2707864240169925No Hit
GGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTT626890.23010532634605596No Hit
AGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGT561270.2060189451391007No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCCCTTGCCT512430.18809180618531077No Hit
TTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGC426730.15663488955263677No Hit
TTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCA404090.14832468427184636No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCC386070.14171028943263064No Hit
TGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCAC368490.13525740034975542No Hit
ACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCC358990.13177034424695025No Hit
AAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAG330280.12123209364573587No Hit
ATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCG326550.11986296530221344No Hit
GCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCC314730.11552433339324952No Hit
GGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCC301590.11070118421526427No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTC299060.10977252611630668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCA36850.039.2028162
GCTACGC38400.037.943471
GCCATAC43250.032.8991252
ACCCTCG45050.031.65348416
GGCCATA50300.028.16475151
GTGCGGC52650.027.37896547
CCCTCGT52550.027.31283417
TCGTGCA54150.027.13558420
GCCGCAA54650.026.60437834
TGCGGCC56050.026.10521548
CGGCCAT57400.025.11310250
TCAACTG59350.024.81003428
CATACCA57450.024.49759954
ACTGCCG60350.024.0395931
CCATACC60500.023.72374753
CCTCGTG62150.023.3932718
ACGCACG64800.022.5324064
GCGGCCA65450.021.92967849
AAGTGCG67400.021.61724945
TGCCGCA69850.021.17007433
Produced by FastQC (version 0.11.2)