FastQCFastQC Report
Tue 11 Nov 2014
141027WaxA_D14-7413_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename141027WaxA_D14-7413_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences187831244
Sequences flagged as poor quality0
Sequence length40
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTGTGACAATGGTGTTTGAGATCGGAAGAGCACACG43728792.328089250156912No Hit
TGGAGTGTGACAATGGTGTTTGTAGATCGGAAGAGCACAC43092102.2941923336247507No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGA36683021.9529775355158696Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGAGTGTGACAATGGTGTTTAGATCGGAAGAGCACACGT29618841.5768856857488522No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCT28877691.5374273941347054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACG25607491.3633243040226044No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT23967231.2759980442870305No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTG23323221.2417114162327543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACAC23166871.2333874549646278No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAAC16226800.8639031321115033Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACG14102070.7507840388897175No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT12606000.6711343507899037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA12171510.6480024164669856Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCA11559390.6154135890193008Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC10886840.5796075119430077No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACT9920620.528166655809403Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAGTGTGACAATGGTGTTTGAAGATCGGAAGAGCACAC8798930.46844868897317216No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGT8317090.4427958747906712No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACT8265320.4400396773180079Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTGA7153460.3808450525941254No Hit
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACG7064720.3761205989776653No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACG6730550.35832962912176636No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACG6602400.35150701552080443No Hit
CTGACCTATGAATTGACAGCCAGATCGGAAGAGCACACGT6494150.345743863571494No Hit
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAA6387240.34005205225601337Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACG6112890.3254458560685463No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTC6054990.3223633018157512No Hit
CGACTCTTAGCGGTGGATCAGATCGGAAGAGCACACGTCT6029030.3209812101334962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTCTTAGCGGTGGATCACTAGATCGGAAGAGCACACG5890420.31360171367443No Hit
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTG5669790.30185553155363226Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAACAGCAACTCCATGTGGAAGATCGGAAGAGCACACG5392360.28708535838691457No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAG5326330.2835699687960327No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACG4965660.26436815804723096No Hit
TGGAGTGTGACAATGGTGTTTGCAGATCGGAAGAGCACAC4203330.22378225850434125No Hit
CTGACCTATGAATTGACAGCAGATCGGAAGAGCACACGTC4122860.2194980937250248No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGT3994430.21266057312594916Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAA3516990.187242011770949No Hit
TGGAGTGTGACAATGGTGTTAGATCGGAAGAGCACACGTC3442860.18329538402034967No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGA3426630.18243131052254544No Hit
CGACTCTTAGCGGTGGATCACTCGGCAGATCGGAAGAGCA3260860.17360583524645132No Hit
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACAC3209440.17086827152143017No Hit
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACA3149810.16769361331600402No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA2997030.15955971627382717Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATC2973990.15833308328618642No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG2958280.15749669421345044No Hit
CTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA2945910.15683812433249922Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGG2943530.15671141484853288No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAA2923980.15567058694452346No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGAT2917920.15534795691392003No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGA2877910.15321785336203173No Hit
TGTAACAGCAACTCCATGTGGAAAGATCGGAAGAGCACAC2854200.15195555005747605No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGT2842440.1513294561367011No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT2791700.14862809512138458Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACA2725080.14508129435590597No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAG2716260.14461172391532476No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACA2702540.14388128100775396No Hit
CGCGACCTCAGATCAGACAAGATCGGAAGAGCACACGTCT2565140.13656620407625047Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAA2455030.130704027068042Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGA2454110.13065504693138272No Hit
CGATACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTC2442310.13002682343944866No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCG2441330.12997464894605074No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAG2439940.12990064634827206No Hit
CGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2420280.12885396212357514Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG2392270.12736272991941638No Hit
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACAC2359920.12564043924449544No Hit
TGACCTATGAATTGACAGCCAGTAGATCGGAAGAGCACAC2293360.1220968328357555No Hit
TACCTGGTTGATCCTGCCAGTAGCAGATCGGAAGAGCACA2281720.1214771276284578No Hit
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACG2275630.12115290041948507No Hit
TACAGTACTGTGATAGCTGAAGAGATCGGAAGAGCACACG2238710.11918730623963711No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACAC2191040.11664938981078142No Hit
CGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAAC2154070.11468113366698461Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAA2140460.11395654708010133No Hit
TGAAAAGAACTTTGAAGAGAGAGTAGATCGGAAGAGCACA2124620.1131132369010983No Hit
CGCCTGTCTGAGCAGATCGGAAGAGCACACGTCTGAACTC2124010.11308076094092205Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCAAGACGGGACTGAAGAGATCGGAAGAGCACACGTC2113710.11253239636745417No Hit
CTGACCTATGAATTGACAGCCAAGATCGGAAGAGCACACG2075780.11051303051583898No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTC2026870.10790909738105127Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCA2022990.10770252897861869No Hit
GACTCTTAGCGGTGGATCACTCGGCAGATCGGAAGAGCAC1993970.1061575251026927No Hit
TGTCTGAGCGTAGATCGGAAGAGCACACGTCTGAACTCCA1980120.10542016108885484Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACG1929940.10274861406976572No Hit
TACGCCTGTCTGAGCGTCGCCAGATCGGAAGAGCACACGT1886180.10041886322171194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAATG16372800.034.0110749
ACAATGG16328450.034.0028510
TGGTGTT15882450.033.97364814
AATGGTG16153400.033.9258812
CAATGGT16233100.033.9081511
TGGAGTG16278550.033.8854641
ATGGTGT16117850.033.85953513
AGTGTGA16384200.033.8584484
GTGTGAC16536600.033.8575635
TGACAAT16448950.033.847118
GATAGGC1044450.033.8230415
CACTACA3589400.033.818857
CAGACTG6149400.033.8162579
GGTGTTT15223150.033.81440715
TCAGTGC3560550.033.8008651
ACAGAAC3736750.033.77613411
AGCTTAT6069550.033.7582972
GTGTTTG11576150.033.74933616
GTGACAA16560600.033.746857
CAACTCC1340250.033.738239
Produced by FastQC (version 0.11.2)