FastQCFastQC Report
Thu 1 Jun 2017
G7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG7.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10343616
Sequences flagged as poor quality0
Sequence length35
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG13900.021.1733913
CTCGTAT13900.020.96468212
GCTCGTA14600.020.05902111
CGTATGC14550.019.63277414
ATGCCGT14700.018.93996217
TATGCCG15150.018.85569416
TGCCGTC16700.016.58479118
AGCTCGT18500.016.22204610
CCGTCTT17750.016.094220
GCCGTCT18150.015.81917419
CGTCTTC19450.014.68601921
GTATGCC20100.014.50034915
GAGCTCG25500.012.3945799
GTCTTCT33500.09.04622422
CGGAAGA43500.07.63285264
TCGGAAG46550.07.2884773
GCTTAGA20600.07.2502829
TTCTGCT45800.06.996994525
TCTGCTT46350.06.820210526
CGGTTCG2609.926882E-46.692662226
Produced by FastQC (version 0.11.2)