FastQCFastQC Report
Thu 1 Jun 2017
G3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9265332
Sequences flagged as poor quality0
Sequence length35
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3550.012.25161712
TCGTATG3600.011.27608713
GCTCGTA3800.011.06323411
TATGCCG4900.09.17204416
CGTATGC5000.08.98860314
ATGCCGT5351.8189894E-127.58756517
TGCCGTC6550.07.304208318
CGGAAGA28050.07.242284329
CCGTCTT6950.07.09272920
GCCGTCT6950.07.092154519
CGTCTTC7900.06.97495521
AGCTCGT7550.06.52825810
ATCGGAA39850.06.481448729
TCGGAAG32550.06.285625529
GGTAAGG14500.06.00182151
GATCGGA48050.05.88872929
AGATCGG58800.05.601816729
TAGATCG24350.05.601563529
GGTAGCA13400.05.52033141
GGTGTAG12400.05.3806651
Produced by FastQC (version 0.11.2)