FastQCFastQC Report
Thu 1 Jun 2017
131127Wax_D13-6250_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6250_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22839469
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCCGGGATCTCGTA252660.11062428815661168Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC37800.034.05350532
TCTCGTA37200.033.66118244
GATCGGA44650.033.5332031
ATGCCGA44500.032.7143225
CCGTCCG40050.032.6869934
CGGTTCA45450.032.2946412
GATCTCG39650.031.91250842
ACCGTCC42050.031.0802633
CGTCCGG42150.031.00652335
AGACCGT41800.031.00428431
ATCGGAA49050.030.9810832
ATCTCGT41050.030.55749543
AGCGGTT48350.030.45103610
CGAGACC43750.030.27287129
GAGCGGT48750.030.2475039
AATGCCG49850.029.51075724
GCGGTTC50200.029.41880211
CGGGATC44900.028.22984539
CCGAGAC47150.028.08988628
TGCCGAG52300.027.7097526
Produced by FastQC (version 0.11.2)