FastQCFastQC Report
Thu 1 Jun 2017
131127Wax_D13-6249_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6249_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences24760591
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA28900.030.4613461
TCTCGTA27050.028.81036244
ATGCCGA30950.027.9384625
TATCTCG28700.027.23029342
ATCGGAA33600.026.9797842
CGGTTCA32600.026.18655612
AGCGGTT34750.024.95632410
CGAGACC32650.024.13711429
ATCTCGT32450.024.01603543
GCGGTTC36500.023.94541211
GAGCGGT36300.023.6418329
AATGCCG37400.023.17872824
CGGGATC33700.023.12523335
AGACCGG34550.022.55630331
TGCCGAG39500.021.835626
CCGAGAC36700.021.77172528
GAGACCG36850.021.62369530
CCGGGAT36600.021.5321534
AAGAGCG42600.021.1741647
GACCGGG37300.020.7172432
Produced by FastQC (version 0.11.2)