FastQCFastQC Report
Thu 1 Jun 2017
131127Wax_D13-6247_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6247_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences25581622
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATCACATCTCGTA734910.2872804546951714Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT92250.038.4660333
TCTCGTA91750.038.41320444
CGCATCA92550.038.2230835
ATGCCGA100850.037.98594325
GATCGGA104600.037.1029971
AGACCGC97100.036.77015731
CCGCATC96300.036.73464234
GACCGCA97250.036.53336332
ATCTCGT96700.036.469543
CGGTTCA104700.036.30607612
ATCGGAA110150.035.8201142
CGAGACC100050.035.6203429
AGCGGTT106900.035.5377710
CATCTCG100700.035.15129542
AATGCCG110450.034.8230524
GAGCGGT109800.034.681449
TGCCGAG110800.034.5747526
CCGAGAC103500.034.32724428
GCGGTTC110750.034.24118811
GAGACCG105200.033.77252630
Produced by FastQC (version 0.11.2)