FastQCFastQC Report
Thu 1 Jun 2017
131127Wax_D13-6246_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6246_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22807963
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGGTGATCTCGTA228170.10003962212671075Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG28000.037.60798633
CGACGGT28550.035.27319335
TCTCGTA30900.033.5824944
CCGACGG32400.032.23045334
ATGCCGA36600.032.1806125
GACCGAC34500.030.90316232
GAGCGGT40200.029.5967149
CGGTTCA40150.029.40865112
GATCTCG35600.029.33333642
AGCGGTT40600.029.13830410
GATCGGA42150.028.8041021
CGGTGAT35650.028.49387438
ATCTCGT36300.028.46612743
AGACCGA37800.028.03151731
CGAGACC38700.027.4927629
GCGGTTC43350.027.28985211
AATGCCG43700.027.05237224
ATCGGAA46050.026.5340942
TGCCGAG44450.026.44816626
CCGAGAC40700.026.03418528
Produced by FastQC (version 0.11.2)