FastQCFastQC Report
Thu 1 Jun 2017
110414Wax_D11-1463_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename110414Wax_D11-1463_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences17642388
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA6450.012.6661981
ATCGGAA8450.09.8621452
ATGCCGA8700.09.57413425
CGGTTCA10250.09.11946212
AGCGGTT9800.08.49712610
AATGCCG10800.08.34142724
GCGGTTC10650.07.81895111
GAGCGGT11350.07.78591259
AGACCGT9650.07.050023631
GAGACCG12950.06.960936530
CGAGACC12250.06.803330429
AAGAGCG13550.06.7726747
CCGAGAC13150.06.72572628
ACCGTGG13500.06.299183433
GACCGTG11200.06.22620932
TTGACGA63300.06.07206962
CTTGACG64400.06.0257861
TGCCGAG13750.05.9358726
TCGGAAG15050.05.8722343
AGAGCGG18650.05.83184058
Produced by FastQC (version 0.11.2)