FastQCFastQC Report
Sun 13 May 2018
LZ_M26.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M26.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18477513
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC4818732.6078888430493863Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTAGGTATAGTAAATGTATTGAATCCATCATA407730.22066281322600073No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG398820.21584073570939988No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTT369910.20019469070323478No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT284940.15420906482382124No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCC283150.15324031973350527No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA271240.1467946470934698No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCT246670.13349740303254015No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTC241920.13092671075376866No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGG230110.12453515795118099No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG228580.12370712443822932No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATC203350.11005268945014407No Hit
CTTTTCTACATTAAAGTTCCTTCCCGTAGAACT187640.10155046298709139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA516050.026.8431971
CGTCTGA520850.026.32356616
ATCGGAA525850.026.3062292
ACGTCTG521550.026.30160315
CGGAAGA528450.026.1862374
GCACACG528850.025.94117211
ACACGTC532000.025.7900413
CACACGT534150.025.69381512
AGCACAC536500.025.6568210
TCGGAAG540300.025.6045593
CACGTCT539900.025.41746514
GAACTCC539700.025.35212121
AGAGCAC547200.025.2177648
GAGCACA550000.025.1031489
AACTCCA546500.025.04002222
CAGTCAC540000.024.87287327
AAGAGCA559500.024.7912457
TCCAGTC552300.024.71733325
ACTCCAG554050.024.71722623
GAAGAGC568650.024.4040056
Produced by FastQC (version 0.11.2)