FastQCFastQC Report
Sun 13 May 2018
LZ_M24.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M24.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17004434
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC2264341.331617388735197Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTAGGTATAGTAAATGTATTGAATCCATCATA329350.19368477657062858No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG283100.16648598830163944No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTT261100.1535481863142284No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCC218960.12876641468925104No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT212700.1250850219419241No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA189920.11168851606586847No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG180720.10627816250749658No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCT177150.10417871009408487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA248400.026.6666741
ACGTCTG253550.025.85880515
CGTCTGA254000.025.79074516
ATCGGAA258050.025.5900542
CGGAAGA259150.025.4808314
GCACACG259800.025.2678211
ACACGTC264250.024.78604113
CACACGT266400.024.61133812
AGCACAC270000.024.41325210
TCGGAAG271650.024.3282783
AGAGCAC273200.024.1359028
CACGTCT272650.024.04716914
GAACTCC275150.023.87752721
GAGCACA278050.023.7210129
AACTCCA278000.023.62365522
CAGTCAC273100.023.48360327
AAGAGCA284250.023.302117
TCCAGTC281300.023.18781725
ACTCCAG282000.023.18291323
GTCTGAA287600.022.84335117
Produced by FastQC (version 0.11.2)