FastQCFastQC Report
Sun 13 May 2018
LZ_M21.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M21.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17558897
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC3532492.01179493222154Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTGGATAGGTCAATGATATTTTCTCTAAGG266880.15199132382859812No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA265210.15104023903095964No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTT249710.1422128052804228No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA205060.11678410096032797No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCT198910.11328160305285691No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT184150.10487560807492635No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCC182950.10419219384907832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA386350.026.7529471
ACGTCTG408450.026.19560415
CGTCTGA409800.026.17848616
CGGAAGA407900.026.0431754
GCACACG415200.025.83461611
ATCGGAA399800.025.7980042
AGCACAC421900.025.51073810
ACACGTC420300.025.48580213
CACACGT420200.025.48223112
TCGGAAG405600.025.4589083
CACGTCT424750.025.21561414
GAACTCC427550.025.10114321
AGAGCAC432100.025.0207238
GAGCACA434100.024.8122259
AACTCCA433500.024.74401922
AAGAGCA442850.024.5749117
CAGTCAC416800.024.49547227
ACTCCAG438950.024.35676823
TCCAGTC437850.024.25798225
GTCTGAA445750.024.10028517
Produced by FastQC (version 0.11.2)