FastQCFastQC Report
Sun 13 May 2018
LZ_M13.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M13.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24999103
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC7445922.978474867678252Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTT314530.12581651429653296No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG313560.12542850037459344No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAG279870.11195201683836416Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA822650.026.7929691
ACGTCTG857950.026.22221215
CGTCTGA857450.026.22170616
ATCGGAA838650.026.2178122
CGGAAGA862900.026.2030894
TCGGAAG846850.025.9347863
GCACACG871550.025.88205711
ACACGTC870650.025.86327713
CACACGT874150.025.76899112
AGCACAC881550.025.65410
CACGTCT880850.025.53880914
AGAGCAC888300.025.503088
GAACTCC882900.025.40000721
GAGCACA898000.025.217139
AACTCCA891400.025.1426622
AAGAGCA908050.025.1104327
CAGTCAC861350.025.01687227
GTCTGAA900400.024.98439817
ACTCCAG899700.024.89270823
TCCAGTC902150.024.76824825
Produced by FastQC (version 0.11.2)