FastQCFastQC Report
Wed 9 May 2018
LZ_M12.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M12.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14380319
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC5274713.6680062521561583Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTT221380.15394651537285092No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT220100.15305640994473071No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA192420.13380788006163144No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA176130.12247989769907051No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT152410.1059851314842181No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG149780.1041562429873774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA558900.026.9348581
ATCGGAA566250.026.5250232
CGGAAGA566550.026.4643544
ACGTCTG565300.026.42011315
CGTCTGA565900.026.3539516
GCACACG573300.026.09144811
TCGGAAG574300.026.0793443
ACACGTC574550.026.01824413
CACACGT574600.026.0159812
AGAGCAC579000.025.909168
AGCACAC578300.025.90785810
CACGTCT579650.025.78001214
GAGCACA585300.025.6187559
GAACTCC582300.025.54593721
CAGTCAC566300.025.53135527
AAGAGCA592700.025.3626427
AACTCCA587650.025.30770722
ACTCCAG590750.025.21145223
TCCAGTC588650.025.17592225
GTCTGAA595850.025.05872317
Produced by FastQC (version 0.11.2)