FastQCFastQC Report
Wed 14 Jun 2017
SRR1178013_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178013_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16094612
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATACG21750.018.34146914-15
ACTAGCC58600.015.9653835
CCACTAG64350.014.8507513
CGCGTAT4502.2573658E-914.7945891
CTAGCCT73250.013.2239666
CCCCACT118000.012.3721081
GATCGTA20900.012.2703865
CACTAGC81650.012.1563974
CCGCAAT14900.012.1278741
CGGCATA33600.011.5895112-13
TAGCCTC84800.011.4787467
CGGCGAT15450.011.0805521
CCGGCAT34100.010.8774631
CGGGCAT39900.010.7264741
GTCGAAC11100.010.2819541
CGACAAT29350.010.207511
AGACGGC88100.09.80901059
CTTAGAT47750.09.7597811
CGAAAAT35100.09.7546741
CGGGCGT14900.09.5746371
Produced by FastQC (version 0.11.2)