FastQCFastQC Report
Wed 14 Jun 2017
SRR1178012_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178012_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18300443
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATACG35650.024.05336614-15
GATCGGG33950.022.1350821
AGACGGC112550.019.9109949
ATCGGGA40450.018.1078112
CGGCATA52050.017.5153112-13
ACTAGCC65200.016.8243375
CTAGCCT86900.015.5987181
CCACTAG76100.015.4231113
GATCGTA23550.015.3248515
GTCGAAC17500.014.9481831
GACGGCA66600.014.75580410-11
GAAGACG173150.013.7103917
TAGCCTC99600.013.3709562
TCGGGGG72100.013.10533238-39
CCCCACT132400.013.0401461
CGCGTTT7750.012.88787651
CGACAAT40600.012.7692171
CAAGCAG200600.012.6137581
CACTAGC90850.012.4417484
AAGACGG189750.012.3606588
Produced by FastQC (version 0.11.2)