FastQCFastQC Report
Wed 14 Jun 2017
SRR1178004_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178004_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19622049
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC204350.10414304846552977TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC82300.020.65712444-45
TATGCCG91050.018.75018746-47
GATCGGA138250.017.9631671
ATCGGAA145200.017.2733152
GTCGAAC19500.016.1022621
CGGAAGA167550.015.1922264
TGCCGTC114400.014.94391148-49
CACACGT116050.014.91546712-13
CACGTCT117400.014.90573114-15
TCGGAAG171650.014.8606883
ATCTCGT118200.014.28374240-41
CGACAAT58650.013.7086981
CTCGTAT125200.013.50315142-43
CGGGTAA13050.012.75954151
GATCGTA32000.012.6143375
TCTCGTA103200.012.05706240-41
ACACGTC106300.011.86084712-13
CGCGGCA16100.011.8198421
ACTAGCC61250.011.707565
GCGGAAT56100.011.7029081
Produced by FastQC (version 0.11.2)