FastQCFastQC Report
Wed 14 Jun 2017
SRR1177985_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177985_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16537337
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2802021.6943598597525102TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA588900.056.3356632
GATCGGA595300.056.0730861
CGGAAGA588700.055.9191324
TCGGAAG599300.055.0410273
AGAGCAC643350.050.558218
GAGCACA675050.048.247369
AAGAGCA716300.045.8202677
GAAGAGC737650.044.5894626
GGAAGAG826400.040.2893455
CGTATGC452650.036.41040444-45
TATGCCG462000.035.77633346-47
AATCTCG447900.035.30134638-39
GACCAAT473100.034.93192334-35
TCGTATG470100.034.71029342-43
CTCGTAT462400.034.6309142-43
ATCTCGT458000.034.61620340-41
TCTCGTA453050.034.47547540-41
TGCCGTC478200.034.4401848-49
ACCAATC475850.034.056336-37
GTATGCC497750.032.87278744-45
Produced by FastQC (version 0.11.2)