FastQCFastQC Report
Wed 14 Jun 2017
SRR1177984_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177984_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15732068
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC142050.046.069529
GAAGACG184000.036.469637
GCATACG70700.034.9088314-15
AAGACGG195350.034.1805468
GATCGGG44750.032.021551
CGGCATA83800.030.25283612-13
ATCGGGA48100.028.4180742
CAAGCAG210600.027.509691
GACGGCA103200.026.64414610-11
CGGCGTG51300.025.91251212-13
AGCAGAA264400.025.7668933
CATACGA91850.025.34610414-15
AGAGCGG74450.023.9844768
ATACGAG93300.023.88090316-17
CGAGATT73500.023.82133318-19
AAGCAGA295050.023.694872
AGAAGAC275800.023.090946
GAGCGGC74350.022.6753039
GGCATAC113200.022.4166212-13
GACGCGT16350.022.23623744-45
Produced by FastQC (version 0.11.2)