FastQCFastQC Report
Wed 14 Jun 2017
SRR1177984_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177984_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15732068
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2250571.430562085035483TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA501900.053.9017752
CGGAAGA499600.053.8498544
GATCGGA506150.053.7776641
TCGGAAG516700.052.08613
AGAGCAC555800.047.563288
GAGCACA591450.044.9453549
AAGAGCA629500.042.3341567
GAAGAGC645700.041.3303646
GGAAGAG729550.037.3131455
CGTATGC379950.035.02881644-45
TATGCCG389600.034.21604546-47
AATCTCG377400.033.5538238-39
TGCCGTC405150.033.0962548-49
CTCGTAT390700.032.95868742-43
TCGTATG395550.032.9388342-43
GACCAAT406550.032.7427734-35
ATCTCGT389800.032.71795740-41
TCTCGTA380450.032.6543440-41
ACCAATC406850.032.2341236-37
GTATGCC423800.030.92809744-45
Produced by FastQC (version 0.11.2)