FastQCFastQC Report
Wed 14 Jun 2017
SRR1177970_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177970_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19120119
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA47800.023.95976846-47
CGTATCA51750.019.7848950-51
GCCGTAT54750.018.96020348-49
CCGTATC63300.017.86277448-49
AGAGCGT128950.017.199329
CGTCGTG81750.016.31836912-13
AAGAGCG143900.015.775538
GATCTCG91650.015.46036934-35
AGCGTCG96700.014.65993110-11
ACTAGCC63250.014.5660245
TCTCGGT93000.013.67727836-37
CTAGCCT91800.013.5411241
GTGGTCG76600.013.43374742-43
TCGTGTA94600.013.320086514-15
GCGTCGT81000.013.30451612-13
CCACTAG74900.013.0569893
TCGGTGG109550.012.99924738-39
GATCGTA29400.012.599325
GTCGTGT88200.012.34945514-15
CGTGTAG93550.011.97152816-17
Produced by FastQC (version 0.11.2)