FastQCFastQC Report
Wed 14 Jun 2017
SRR1177969_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177969_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19310282
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC65850.020.1970644-45
TATGCCG78900.017.18759246-47
CTAGCCT94250.016.0262411
CGATGTA85850.015.65784634-35
ACTAGCC68800.015.5338825
TCACCGA90350.015.24599430-31
TATCTCG77900.014.90827438-39
GATCGTA28000.014.5890265
CACCGAT84550.014.43794630-31
CCACTAG77450.014.2282833
TGCCGTC97750.014.09182148-49
CCGATGT82250.013.97543532-33
ATCGGAA176950.013.5021622
GATCGGA179300.013.35168651
CGGAAGA190000.013.0747684
GTCGAAC18750.012.919771
GTCACCG97850.012.49977928-29
TAGCCTC116300.012.3342612
ATCTCGT110050.012.32258940-41
GGATCGT38600.012.1824264
Produced by FastQC (version 0.11.2)