FastQCFastQC Report
Wed 13 Jul 2016
SRR891274_GM12878_ATAC_CHROMATIN_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR891274_GM12878_ATAC_CHROMATIN_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24184246
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT1432320.5922533206121042Illumina Single End PCR Primer 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT162900.040.2199744
TCGGTGG171750.039.84066443
CTCGGTG173200.039.49505642
GATCTCG210250.033.08119639
ATCTCGG215650.032.24327540
AGATCTC216900.032.12522538
TAGATCT216700.031.96497237
GTAGATC216600.031.93950536
TCTCGGT221500.031.35217741
CGAGTGT248350.027.32063331
CCGACGA255600.027.24882127
GACGAGT250600.027.24866729
CGACGAG256250.027.24073828
TGCCGAC257950.027.11060525
GCCGACG258800.027.03035726
CTGCCGA260200.026.8841424
TGACGCT267550.026.70995319
ACGCTGC265200.026.49904821
CGCTGCC267100.026.38451422
GCTGCCG267600.026.31406823
Produced by FastQC (version 0.11.2)