FastQCFastQC Report
Tue 12 Jul 2016
SRR891273_GM12878_ATAC_CHROMATIN_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR891273_GM12878_ATAC_CHROMATIN_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22898819
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA362220.15818283030229638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA53300.032.1093544
GCGAATC54950.031.18726739
CGAATCT55750.030.85609640
ATCTCGT56550.030.18618443
GGCGAAT69600.026.7088438
AATCTCG65350.026.39061742
ACGAGAC71700.025.52619628
CCCACGA76450.024.02646825
CGAGCCC77300.023.79322621
GACTAAG79800.023.0457132
TCCGAGC80150.023.00197419
ACTAAGG83900.022.05186733
AGGCGAA88450.021.48935937
GAATCTC82750.021.02749841
GCCCACG91050.020.19795224
CGAGACT94100.020.05762929
CCGAGCC93300.020.04296320
CCACGAG94200.019.52254126
TCTCCGA96400.019.14738817
AGACTAA98200.018.92918431
Produced by FastQC (version 0.11.2)