FastQCFastQC Report
Sun 6 Aug 2017
G150-M21-Index-22_CGTACG-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M21-Index-22_CGTACG-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24399864
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG7350.028.70112233
CGTACGT8300.025.15161134
ACCGTAC10550.020.82854732
CGTAATC10550.019.99093838
GATCGGA11200.019.2829691
GTACGTA12100.017.79685235
CTCGTAT12800.017.16499144
TCTCGTA12900.016.69009243
AATCTCG13200.016.47636441
CACCGTA13850.015.70709731
ATCGGAA14300.015.2456652
TACGTAA15350.014.45779836
ACGTAAT15950.013.77498237
ATCTCGT16700.012.7600642
CGTCTGA19200.012.27104616
ACACGTC18600.011.95664213
GTCACCG19800.011.20835229
TCACCGT20900.010.93399530
ACGTCTG22050.010.78418715
TCGGAAG22650.010.4027373
Produced by FastQC (version 0.11.2)