FastQCFastQC Report
Sun 6 Aug 2017
H3K27ac_Veh_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3K27ac_Veh_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61305288
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT45050.024.21789442
TCGTATG46550.024.09759943
TCTCGTA45400.023.06194941
CGTATGC50700.022.16889444
GATCGGA53300.022.0509821
ATCGGAA54250.021.5285382
ATCTCGT55450.018.881740
ACACGTC69550.016.82752813
CGTCTGA69600.016.75018916
ACGTCTG80200.014.6459715
TCGGAAG88500.013.6414083
CACGTCT94400.012.63008214
CGGAAGA101750.011.8006254
CACACGT118950.010.50484712
CATCTCG60900.010.14945639
CGATGTA26950.09.55151534
GCACACG135300.09.54433711
TATCTCG28500.09.107326539
ACCGATG35150.08.19937732
CACCGAT34150.08.11734631
Produced by FastQC (version 0.11.2)