FastQCFastQC Report
Sun 6 Aug 2017
G150-M6-Index-6_GCCAAT-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M6-Index-6_GCCAAT-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19158965
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG21250.030.545612
GCGTCGT21700.030.01356111
AGCGTCG22900.028.82513210
TCGTGTA24500.026.58267814
GATCGGA25050.026.3473361
ATCGGAA24800.026.2578892
GTCGTGT26400.024.75319513
CGTGTAG26550.024.69602815
GATCTCG23850.022.22831534
TGGTCGC23450.021.39094442
AGAGCGT31700.020.9616998
GAGCGTC31700.020.8925069
ATCTCGG27500.019.2780135
GTGGTCG27000.019.1463741
TCGGAAG36550.018.478723
AAGAGCG37200.017.9806757
GTCGCCG31000.016.60829444
CGGAAGA40750.016.5209564
TCTCGGT32850.016.13836536
GGTCGCC34200.015.3752943
Produced by FastQC (version 0.11.2)