FastQCFastQC Report
Wed 24 Apr 2019
SRR5517163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5517163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43167615
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT37142758.604309040469342TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG12243732.8363230166873943TruSeq Adapter, Index 7 (97% over 34bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT886690.20540629821684614TruSeq Adapter, Index 9 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTAACTCGTATG799750.18526620013637538TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)