FastQCFastQC Report
Wed 24 Apr 2019
SRR5517159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5517159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48962775
Sequences flagged as poor quality0
Sequence length50
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG6916151.4125322757952343TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT4515130.9221556580483847TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAACTCGTATG619550.12653490330153877TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)