FastQCFastQC Report
Wed 24 Apr 2019
SRR5517158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5517158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46087698
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT12515902.7156704593924395Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC2205150.47846824547409594Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCC656370.14241761434906122Illumina Single End PCR Primer 1 (96% over 32bp)
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC467060.10134157709504171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)