FastQCFastQC Report
Tue 23 Apr 2019
G168_G169_M5_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG168_G169_M5_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29013815
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC677610.2335473635576707TruSeq Adapter, Index 7 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322540.11116773164783743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)