FastQCFastQC Report
Tue 7 May 2019
SRR8168086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41524626
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG103050.024.9791473
ATGCCGT163100.016.5032234
CGTATGC159650.016.3547761
GCCGTCT179650.015.4013876
TGCCGTC196150.013.89059455
CCGTCTT209700.013.1947527
CTCCGAT143200.012.7141851
CTCGAAT151800.012.3967761
TCTAACG31450.012.2484962
GTATGCC217700.011.8684452
GCATATC122000.011.8593996
AACGCGT71400.011.8418453
TCCGATT110750.011.45274452
CCGATTA92050.011.4306073
ATATCAC128050.011.3364578
CTAACGC78200.011.1899141
CCACTAG156850.010.6613083
CGTCTTC267250.010.6173888
ATGCATA140350.010.5765194
TAACGCG78950.010.4724932
Produced by FastQC (version 0.11.2)