FastQCFastQC Report
Tue 7 May 2019
SRR8168085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32130794
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT396470.12339253116496281No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT373830.11634633118621344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA141400.027.7724592
CACGATC150850.027.0434191
CGATCAC150650.026.1572783
CCACGAT128900.024.4208621
TCACAAG254850.015.8127146
ATTGGAC294900.014.0957356
CTCGAAT86100.013.0611851
CGCCATT72950.012.5775811
GAATTGG375400.012.4141234
CGCGTAT9550.012.31755351
AATTGGA375500.012.235435
TAATGTA287900.012.1035614
CTGGAAT548600.012.1020531
GGAATTG423250.011.9101023
ATCACAA372700.011.6207685
CACAAGA361050.011.5263987
AAGACGT307000.011.25906114-15
CCCGGTT49000.011.1390911
CGATTGG281300.011.0835894
TTGGACT384150.011.0536227
Produced by FastQC (version 0.11.2)