FastQCFastQC Report
Tue 7 May 2019
SRR8168083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34388359
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN549610.15982443361138576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC93750.018.7073753
ACGATCA102100.017.1332842
CACGATC123900.015.6066521
CCACGAT110550.013.4908641
CCGATTA58500.013.26182653
TCCGATT73550.012.5313182
AACGCGT55650.012.5046583
CTCCGAT115600.012.1689351
CTCGCAT74250.012.1658941
CCCGGTT52050.012.0218161
CTCGAAT99550.011.9096361
CTCGTCT126950.011.859231
CTAACGC62600.011.7995251
ATTGGAC157900.011.5540876
TCTAACG25300.011.1520352
CTCGGTT86550.011.1436131
TTACGAC26250.011.10625
GTGCGCG51500.010.9567628
CTCGTGT133650.010.5606711
GCATATC90400.010.5587786
Produced by FastQC (version 0.11.2)