FastQCFastQC Report
Tue 7 May 2019
SRR8168081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32819600
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN378140.11521773574327536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG36800.031.2188853
TACGGCG39200.028.8306646
ACGATCA125450.024.3062612
CGATCAC127300.024.0906493
CACGATC136150.023.4583191
CCACGAT125000.021.0042611
GATACGG58300.020.1086334
ATACGGC66100.017.9491845
ACGGCGA70300.017.210737
ATTGGAC243850.014.865536
CGATCTA28300.014.76370852-53
GGCGACC85100.014.383899
CGGCGAC87600.014.3476968
TCACAAG235650.013.4922976
ACACGAC42850.013.41795338-39
TACACGA40500.013.32735138-39
GAATTGG324250.012.7555654
CTCGAAT93050.012.3667421
CTGGAAT512300.012.29447651
AATTGGA324500.012.08145
Produced by FastQC (version 0.11.2)