FastQCFastQC Report
Tue 7 May 2019
SRR8168079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35175786
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG77450.015.2378123
CTCGAAT137850.012.2180521
CTCCGAT130750.012.0539341
CCGATTA74150.011.8577083
AACGCGT41650.011.6276663
GCATATC97100.011.5735756
CTCGCAT60100.011.4288921
TCCGATT91950.011.353242
CTTAGAT164700.011.3116851
ATATCAC103350.011.2838338
CTCGTGT141650.011.2592331
CTCGTTT125850.011.2148561
CTCGGTT85550.010.9435631
CTCCGGT68200.010.761331
CGATTAG96150.010.6125494
TCGGTTA40650.010.5268732
CGCCAAT45700.010.5004931
TTCGATA37950.010.160632
CCCGAAT123250.010.1536081
CGTATGC117250.010.1515731
Produced by FastQC (version 0.11.2)