FastQCFastQC Report
Tue 7 May 2019
SRR8168078.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168078.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33144325
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1140290.3440377802233113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG87700.019.717033
CGTATGC122000.014.3892981
ATGCCGT135350.013.6787494
CTCGTTT101250.012.4110021
GCATATC86350.011.9720016
TGCCGTC165450.011.5311235
CTCGTGT132000.011.3025381
CTCCGAT123700.011.2999731
ATATCAC93600.011.1455788
CTCGCAT48400.011.0853671
GCCGTCT175350.011.0139336
CCCGAAT116800.011.000451
CCACTAG112000.010.9919953
GTATGCC161550.010.79194452
CCGATTA69800.010.7037253
CTCGAAT125300.010.5546991
CTCGGTT77150.010.5536041
CTTAGAT140600.010.4773061
TTCGATA33050.010.3796842
CCCGGTT57150.010.3763571
Produced by FastQC (version 0.11.2)