FastQCFastQC Report
Tue 7 May 2019
SRR8168077.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168077.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34489953
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG122450.032.0108953
CGTATGC160200.024.5331941
ATGCCGT170250.024.2109494
GCCGTCT196950.021.2152866
TGCCGTC204550.020.4043275
CCGTCTT218000.019.2315947
GTATGCC212200.018.4515042
CGTCTTC250200.017.057588
CTCCGAT122400.012.6515761
CCGATTA71200.012.2778833
CGATTAG95700.011.8850984
AACGCGT55450.011.7815863
CTCGCAT68850.011.6218471
TCTAACG25400.011.2882932
TCCGATT87450.011.2873412
CTCGTTT125550.011.1719681
GCATATC96450.010.9643636
CTCGAAT130800.010.8674921
TTCGATA36850.010.8421042
ATATCAC102050.010.5473028
Produced by FastQC (version 0.11.2)