FastQCFastQC Report
Tue 7 May 2019
SRR8168074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32714570
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG71000.021.0426563
CGTATGC112950.013.4555941
ATGCCGT120850.013.4516394
GCATATC94600.012.664586
GCCGTCT134400.012.444936
TTCGATA38550.012.31022552
CGCGTAT10850.012.142691
ATATCAC100450.011.8340388
TGCCGTC138100.011.7374885
TCGGTTT94750.011.1080482
CCCGGTT55550.011.0961891
CTCGAAT108300.011.0354961
CTCCGAT118350.011.0128091
CCGATTA76200.010.8514923
ATGCATA113450.010.8503194
TCGATAA36000.010.8319683
CTCCGGT61800.010.7353751
CCACTAG129650.010.7263253
CCGTCTT157250.010.6368187
TCGGTTA41000.010.5432132
Produced by FastQC (version 0.11.2)